normal human bronchial epithelial cells beas2b  (ATCC)

Journal: Oncotarget

Article Title: Cancer cell-selective, clathrin-mediated endocytosis of aptamer decorated nanoparticles

doi: 10.18632/oncotarget.24772

Figure Lengend Snippet: Cells were incubated with 50 nM S15-APT QDs for 2 h. Nuclei were stained with 2 μg/ml Hoechst 33342. Fluorescence confocal microscopy was performed using an inverted confocal microscope (Zeiss LSM 710) at ×630 magnification. ( A ) Human A549 non-small cell lung carcinoma cells; ( B ) Normal human bronchial epithelial BEAS2B cells which served as normal non-target cells, ( C ) Human colon adenocarcinoma CaCo-2 cells; ( D ) Human cervical carcinoma HeLa cells; ( E ) A549 cells were incubated with 50 nM S15-APT QDs along with 5 μM free APT; ( F ) A549 cells were incubated with 50 nM random sequence APT-QDs; ( G ) A549 cells incubated with no S15-APT QDs; H) A549 cells incubated with 50 nM Qdot ® 655; ( I ) ABCG2-overexpressing MDR subline A549/K1.5 cells incubated with 50 nM S15-APT QDs; the red fluorescence channel was defined between 10-100 for all presented images.

Article Snippet: Normal human bronchial epithelial BEAS2B cells were generously provided by Prof. Rotem Karni (The Hebrew University, Jerusalem, Israel).

Techniques: Incubation, Staining, Fluorescence, Confocal Microscopy, Microscopy, Sequencing